Generation Of Bone Marrow
BM cells were harvested from femurs of mice, and 2 × 106 cells were cultured, as previously described . The cells were cultured in the presence of 10 ng/ml IL-3 and 50 ng of recombinant murine SCF , and the nonadherent cells were passaged every 3 days. Four weeks later, the cells were used as MCs for experiments . The MCs were identified and counted in three different ways: light microscopy: we counted the MCs with a hemocytometer and used trypan blue to detect any dead cells flow cytometry: BMMCs were stained with FITC rat anti-mouse CD117 antibody, and the percentage of MCs was analyzed with flow cytometry. CD117 is the cKit receptor present on mast cells and toluidine staining . We counted five fields of toluidine-positive BMMCs generated from BM derived from each mouse.
Can Osteoporosis Lead To Bone Cancer
Certain cancer treatment can increase the risk for osteoporosis in both men and women. Osteoporosis after Chemotherapy is usually common as chemotherapy used for breast cancer can cause a loss of bone density. In addition to that, certain hormone therapies for breast and prostate cancer can also lead to bone loss.
However, a number of strategies can help prevent and treat osteoporosis. Thus, we can say that osteoporosis doesnt lead to cancer, instead Bone cancer leads to the osteoporosis condition.
Significant Increase In Primary Tumor Burden As Well As Lung And Bone Metastasis In The Arthritic Versus Nonarthritic Mice With Breast Cancer
We first demonstrated that the 4T1 tumor burden is significantly higher in the arthritic SKG versus the nonarthritic Balb/C mice .1A). Second, we substantiated this finding in the PyV MT mice with higher tumor burden in the arthritic PyV MT versus the nonarthritic PyV MT mice .1B). Third, metastases to the lungs and bones were compared. We report that nine of 10 SKG mice showed lung metastasis, whereas only four of 10 Balb/C mice showed metastasis .1C). Similarly, seven of 10 arthritic PyV MT mice showed lung metastasis, whereas only three of 10 nonarthritic Py VMT mice showed the same .1C). Similar trends were observed with bone metastasis, with eight of 10 SKG mice developing bone metastasis, whereas only three of 10 Balb/C developing the same .1D). Likewise, five of 10 arthritic PyV MT mice developed bone metastasis, whereas none of the nonarthritic PyV MT mice developed bone metastasis .
Higher tumor burden coupled with higher metastasis in arthritic versus nonarthritic breast cancer-bearing mice. Significant increase in tumor burden in SKG versus Balb/C and arthritic PyV MT versus PyV MT mice. Percentage of lung and bone metastasis, respectively. Light-microscopic images of circulating tumor cells cultured from peripheral blood of arthritic versus nonarthritic tumor-bearing mice. .
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What Happens To Patients With Osteoarthritis
Osteoarthritis is slowly progressive, although its course may be non-linear. Predicting incident osteoarthritis or future osteoarthritis course for individual patients is hard, although risk factors are known. In patients with osteoarthritis of the knee, knee pain, presence of Heberden nodes, and malalignment increase the risk for radiographic progression of knee osteoarthritis. Age, ethnicity, body mass index, comorbidity count, joint effusion and baseline osteoarthritis severity are associated with clinical progression of knee osteoarthritis.
In hip osteoarthritis, age, baseline joint space width, femoral head migration, femoral osteophytes, bony sclerosis, and baseline hip pain predict progression of radiographic and/or clinical hip osteoarthritis.
Some studies suggest that osteoarthritis patients show increased mortality as compared to non- osteoarthritis controls, primarily due to cardiovascular disease.
Western Blot And Antibodies
We performed BCA assay to load equal quantities of tumor lysates onto sodium dodecylsulfate-polymerase chain reaction gels. SCF and -actin antibodies were used at 1:200 dilution and were used according to manufacturer’s recommendations.
The densitometric analyses of immunoblots as shown in Additional file 1 were performed by using NIH Imaging program. Results are presented as mean values of arbitrary densitometric units corrected for background intensity and normalized to the expression of -actin.
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How Are Weakened Bones Treated
Medications known as bisphosphonates and monoclonal antibodies can also strengthen your bones and reduce the risk of fractures. We have more information on bone-strengthening drugs.
If a bone is very weak, you may need surgery to strength it. This is done in hospital under a general anaesthetic.
Your surgeon will put a pin into the centre of the weakened bone and might fix a metal plate to hold the bone firm as well. You will need to stay in hospital for a week or so to recover after this surgery.
Spinal cord compression
If cancer has spread to the bones of the spine , it can cause pressure on the spinal cord. This is called spinal cord compression. Its important to get treatment straight away if you have cancer and you notice numbness and loss of power in your legs. Read more about spinal cord compression.
Too much calcium in the blood
Breakdown of bone cells can release extra calcium into your blood. A high calcium level in your blood is called hypercalcaemia. This can make you feel thirsty, sick, constipated or drowsy. It is very important to tell your doctor immediately if you have any of these symptoms.
Lymphoma Linked To Tnf Inhibitors
There have been two cases of RA patients developing a rare and fatal type of non-Hodgkin’s lymphoma called hepatosplenic T-cell lymphoma while taking Humira , a TNF inhibitor. The FDA also
reported 40 other cases in patients with Crohn’s disease and ulcerative colitis who were taking Humira, as well as other TNF inhibitors and immunosuppressantsall of which are also prescribed for RA. Although the agency says it is difficult to know the risk of these drugs because the conditions on their own are associated with increased lymphoma risk, it says the risks and benefits should be “carefully weighed when prescribing these drugs to children and young adults.”
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How To Strengthen Bones After Chemo
Following mentioned methods if followed properly can help to strength bone after chemotherapy:
- Eat a variety of healthy food everyday including a lot of healthy fruits and vegetables
- If you are overweight, get enough calories and protein to reach a healthy weight
- Eat foods that are rich in calcium such as low dairy foods, dark green-leafy vegetables, canned fish, as calcium is an essential nutrients for maintaining bone mass and density.
- Get a good amount of vitamin D. Vitamin D sources include fatty fish, milk such as soy milk, rice milk, and sunlight, etc.
- Limit alcohol and smoking
- Prevent falls and be physically active
How Do Doctors Diagnose Bone Cancer
Whether the cancer in bone is primary or metastatic, the early symptoms vary from no symptoms at all to severe bone pain. It is very common for cancer in bone to not cause any symptoms. This form of cancer can only be detected using imaging tests, such as X-ray tests, computerized tomography , or magnetic resonance imaging . For some cancers doctors will use a general screening method to search all bones for cancer using X-ray screening or nuclear medicine bone scan to find cancer in bone that is not causing symptoms.
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Scf Expression On The Tumors
To confirm that MC activation and proliferation in the tumors and sites of metastasis may be mediated by tumor-derived SCF, we assessed the expression of SCF on 4T1 and PyV MT tumors in vivo by Western blotting. We observed that SCF was highly expressed by the 4T1 and PyV MT tumors in vivo .6A). No difference was found in SCF protein expression levels between tumors derived from arthritic versus nonarthritic mice, indicating that the arthritic milieu does not influence SCF levels. SCF expression was also confirmed on 4T1 and PyV MT tumor cell lines in vitro by IF .
The expression of stem cell factor on the 4T1 and PyV MT cells in vivo and in vitro. Western blotting showing the presence of SCF on the tumors derived from experimental mice, as indicated in the figure . Immunofluorescence confirming the presence of SCF on the 4T1 and PyV MT cells in vitro.
Arthritis Drug May Help Stop The Spread Of Bone Cancer
Human Osteosarcoma Cells Image Credit: Wellcome Images
New research from the University of Queensland Diamantina Institute reveals how an existing treatment for rheumatoid arthritis could be used to prevent the spread of osteosarcoma tumours.
Osteosarcoma accounts for 56% of all malignant bone cancers and 6% of childhood and adolescent cancers. The prognosis depends heavily on whether or not the cancer has spread at the time of diagnosis. If the tumour is still localised at the bone, the 5 year survival is around 70% with treatment. But once the cancer has begun to spread, or metastasise, the 5 year survival drops to around 20%. These patients dont respond well to standard treatment.
We still know very little about the cellular and molecular changes that cause an OS tumour to spread and become life threatening. UQDI researcher Dr Liliana Endo Munoz explains that to understand this, we need to take a closer look at the diversity among the different cancer cells within a tumour.
Any OS tumour is very heterogeneous and we know there are cells that are metastatic and cells that are non-metastatic and everything in between, she explains.
There is quite a lot of genetic diversity among tumour cells within the same tumour. We also know that in a number of other cancers, this genetic variation contributes to some cells becoming resistant to therapy or acquiring the ability to spread.
What we found is that they are all metastatic, but with different abilities.
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Symptoms Of Bone Cancer
Increase your understanding of bone cancer symptoms here.
Symptoms of primary bone cancer can be very general and often appear similar to the symptoms of sports injuries, growing pains or many other common conditions such as tendonitis or arthritis. They may also vary depending on the location and the size of the tumour.
The most common symptoms of primary bone cancer are:
- Bone pain
- This can be constant or intermittent . Painkillers may not help and the pain can be worse at night
- The area may be tender to touch
- A lump or swelling may be seen or felt if the tumour is near the surface of the body. In other places a lump or swelling may not be visible
- Inflammation and tenderness over the bone
- Problems with mobility such as stiff joints or reduced movement
- Development of an unexplained limp, joint stiffness and reduced range of motion
- Easy bruising
- Weight loss
- Loss of muscle tone
- Fracturing of the bone may occur due to the tumour weakening the area . This is known as a pathological fracture
If you are concerned about bone cancer symptoms, please seek advice from your doctor. Please also refer to our Primary Bone Cancer Flashcard.
Symptoms awareness video
Version 2 produced September 2016Information is currently under review
Significant Decrease In Differentiation Of Mast Cells From Bone Marrow Precursors From The Mice Treated With Anti
To determine the effect of the therapy on MC differentiation, we plated 2 × 106 cells from each treatment group of mice. After about 30 days of culture with IL-3 and SCF, we observed a significant decrease in MC population from the BM of mice treated with anti-cKit and anti-SCF ,D), indicating that the BM cells destined to differentiate to MCs are affected by this therapy.
. Significant reduction in the differentiation of BMMCs in mice treated with neutralizing stem-cell factor or cKit antibodies . Representative images of BMMCs showing diminution of MC population in treated group . Significant reduction in migration of 4T1 cells toward MCs from mice treated with anti-SCF or anti-cKit .
Next, we performed a migration assay by keeping the 4T1 cells in the upper chamber and MCs derived from the mice with and without treatment in the lower chamber. We saw a significant decrease in migration of 4T1 cells toward the MCs derived from the mice treated with anti-cKit and anti-SCF .9E). This finding illustrates that this therapeutic intervention not only reduces the differentiation of MCs from BM precursors but also affects migration of the tumor cells toward those MCs. In all instances, treatment with anti-c-Kit was significantly better than that with anti-SCF. The reason for this is unknown, but one can speculate that the anti-c-Kit antibody may have a superior neutralizing effect.
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Can Osteoporosis Be Cured
This question generally occurs in every osteoporosis patients mind that Can Osteoporosis Be Cured.
The main goal of treatment of osteoporosis is the prevention of bone fracture by reducing bone loss, by increasing bone density and strength. There are certain lifestyle changes and dietary changes that one can follow for preventing the bone loss which we have discussed earlier.
In addition to that, certain medications can also help to increase the bone strength and mass.
Treatment for bone loss often involves the use of bone-modifying products that helps to slow the rate of bone thinning. They may also help to reduce new bone damage and promote healing. One of the best bone modifying product available in the market in India is Osteofem.
Marketed by Aldan Healthcare, a Gynecology Pharma Company in India, Osteofem is considered as the best Osteoporosis Treatment in India. It has been made equipped with plant-based polyphenols, effective in increasing bone formation, decreasing bone resorption and augmenting bone strength.
So, here ends the blog for Can Osteoporosis Lead to Bone Cancer.
We hope that you enjoyed reading this blog. If you find this blog interesting then share this with your loved ones.
Till then, Stay Tuned.
How Is Bone Cancer Diagnosed
To diagnose bone cancer, your healthcare provider will often first use X-rays to view images of your bones. Magnetic resonance imaging and CT scans provide more detailed images of the areas around the bones and are usually obtained before any treatment.
To confirm the diagnosis, your healthcare provider will perform a biopsy, where a small piece of tissue is removed from the bone to be examined under a microscope. A biopsy provides specific information about the cancer, including where it formed. Having this information helps providers know which course of treatment will work best for the specific cancer.
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Osteoporosis And Breast Cancer
A women having Breast cancer are at higher risk of developing Osteoporosis. Following mentioned are some of the reasons:
Certain chemotherapy treatments used for breast cancer treatment can cause the ovaries to stop making estrogen that brings on menopause. Moreover, early menopause may also result when the ovaries are removed or if one have radiation to the ovaries. These procedure are done to slow breasts cancer growth because estrogen can cause some breasts tumors to grow. But this sudden lowering of the estrogen levels causes rapid bone loss.
Some chemotherapy medicines may cause a loss of bone density.
In breast cancer, the hormones estrogen and progesterone can speed up the tumor growth. Hormone therapies are used to prevent this from happening, but that can speed up the bone loss, too. Hormone therapy may include ovary ablation or taking medications.
Severe Arthritis Linked To Cancer Risk
But Rheumatoid Arthritis Treatments Don’t Raise Risk, Study Shows
Feb. 27, 2006 – Rheumatoid arthritis patients with the most severe disease are roughly 70 times more likely to develop lymphoma, a type of cancer, than patients with mild disease, according to new research from Sweden.
The findings provide some of the best evidence yet linking the elevated lymphoma risk among rheumatoid arthritis patients to the disease itself and not the drugs used to treat it.
In fact, researchers suggest that the drugs Enbrel, Remicade, and Humira and other new and old treatments for RA may actually reduce the chances of developing lymphoma in high-risk patients by reducing inflammation.
“Conventional medical treatment to suppress and alleviate disease activity is not by itself a risk factor for lymphoma,” they write in the March 2006 issue of Arthritis and Rheumatism.
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How Is Bone Cancer Treated
Bone cancer treatment depends on the type of cancer, whether it has spread and if so, where. People with bone cancer often work with a team of healthcare providers to treat the condition. This group includes doctors who specialize in cancer and doctors who specialize in bones and joints .
Bone cancer treatment typically involves a combination of approaches. The type and duration of these treatments vary depending on several factors, including the type of bone cancer, the size of the tumor and whether it has spread to other parts of the body. The treatments most commonly used include:
- Surgery: Your surgeon removes the tumor and some healthy tissue around it. They can also repair or rebuild affected bones with real or artificial bone grafts. Sometimes, an entire limb must be removed to treat cancer. In this case, an artificial limb can be used. Sometimes repeat surgery is needed if all of the cancer cells were not removed the first time around.
- Radiation therapy: This treatment shrinks the tumors with high doses of X-rays. Healthcare providers often use radiation before surgery to shrink the tumor so less tissue has to be removed.
- Chemotherapy: This type of treatment kills cancer cells throughout the body with medicine. People usually receive this medicine by swallowing a pill or having it injected into a vein. Your provider can use chemotherapy to treat primary bone cancers or bone cancers that have spread.
What Are Common Bone Cancer Symptoms
Some people with bone cancer have no symptoms other than feeling a painless lump. For others, a variety of symptoms can develop. These symptoms may also occur because of other conditions, such as arthritis or Lyme disease, which may delay the diagnosis. The most common signs of bone cancer include:
- Pain .
- Unexplained swelling.
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